A research team led by Kim Pyong Hun, a section head at the General Assay Office, has developed a device for rapidly determining the concentration of biochemical indexes in human blood and other body fluids by spectrophotometric method.
The automated biochemistry analyzer is a medical analytical instrument for diagnosing several kinds of diseases by rapidly determining the concentration of biochemical indexes of blood and other body fluids such as GOT, GPT, TP, CK, GLU, LDH, etc.
It consists of sample injection unit, regeants injection unit, reaction and optical monochromator unit, stirring unit, washing unit, power supply unit, control unit, etc.
In the sample injection unit, the range of sample setting volume is 1 to 20μL with 0.1μL stepping. The sample disk has a total of 120 positions. The sample probe works above the reaction cuvette, and then returns to the sample probe rinse trough.
In the regeants injection unit, the range of reagent setting volume is 10 to 400μL with 1μL stepping. The reagent disk has 80 positions in total. The reagent probe works in the same way as the sample probe. Reagent bottle volumes are 15ml, 20ml, 70ml, etc.
In the reaction and optical monochromator unit, reaction cuvette volume is about 150～500μL. The range of wavelength in the photometric system is 340～750nm. The wavelength accuracy is ±2nm. The light source is a 20W/12V life quartz halogen lamp. The measurement range is 0 to 4Abs. The incubation bath temperature is 37±0.1℃. The reaction disk has 80 positions in all.
In the stirring unit, stirring is carried out after addition of sample and reagent each. After stirring, the stirring rod comes back to the rinse trough.
In the washing unit, the reaction cuvettes are washed after measurement.
In the reagent cooling unit, all reagents are kept at 5~15℃.
In the power supply unit, power is supplied to the signal amplifier and controller as well as to the probe for injecting liquor necessary for sample injection, reagent injection, washing, etc. and to the switching valves for selecting liquor.
The analysis methods include rate assay, end-point assay and 2-point assay. The calibration methods include 1-point linearity, 2-point linearity, multi-point linearity and non-linearity.
The automated biochemistry analyzer is applicable to the following indexes: GOT 35.4~56.3u/L, GPT 25.6~42.3u/L, TP 4.54~5.66mg/dL, CK 115~173u/L, GLU 76.4~106mg/dL, ALB 2.54~4.06mg/dL, LDH 152~214u/L, etc.
The accuracy of the analyzer is ≤1.5% for biochemical indexes.
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